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human normal liver cell line lo2  (ATCC)


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    ATCC human normal liver cell line lo2
    Human Normal Liver Cell Line Lo2, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human normal liver cell line lo2/product/ATCC
    Average 94 stars, based on 52 article reviews
    human normal liver cell line lo2 - by Bioz Stars, 2026-03
    94/100 stars

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    Validation of UbRGs expression was based on HPA database and experiments. (A) The expression profiles of UbRGs in normal and tumor tissues were retrieved from the TCGA database, and the immunohistochemistry images of UbRGs were obtained from the HPA database. (B) RT-qPCR was used to measure the expression levels of BOP1, CDC20, and UBE2S genes in the normal liver cell line (LO2) as well as in liver cancer cell lines (HepG2, Huh7, MHCC97H). (C) Western blotting (WB) was performed to detect the protein levels of BOP1, CDC20, and UBE2S in the normal liver cell line (LO2) as well as in liver cancer cell lines (HepG2, Huh7, MHCC97H). (D) The bar graph illustrates the expression differences of UbRGs among different cell types. (E) Immunofluorescence was used to determine the subcellular localization of UbRGs in tumor cells.

    Journal: Journal of Cancer

    Article Title: Prognostic and Immune Landscape Analysis of Ubiquitination-related Genes in Hepatocellular Carcinoma: Based on Bulk and Single-cell RNA Sequencing Data

    doi: 10.7150/jca.93425

    Figure Lengend Snippet: Validation of UbRGs expression was based on HPA database and experiments. (A) The expression profiles of UbRGs in normal and tumor tissues were retrieved from the TCGA database, and the immunohistochemistry images of UbRGs were obtained from the HPA database. (B) RT-qPCR was used to measure the expression levels of BOP1, CDC20, and UBE2S genes in the normal liver cell line (LO2) as well as in liver cancer cell lines (HepG2, Huh7, MHCC97H). (C) Western blotting (WB) was performed to detect the protein levels of BOP1, CDC20, and UBE2S in the normal liver cell line (LO2) as well as in liver cancer cell lines (HepG2, Huh7, MHCC97H). (D) The bar graph illustrates the expression differences of UbRGs among different cell types. (E) Immunofluorescence was used to determine the subcellular localization of UbRGs in tumor cells.

    Article Snippet: Human liver cancer cell lines (HepG2, Huh7, and MHCC97H) were purchased from ServiceBio, and human normal liver cell lines (LO2) were purchased from iCell Bioscience.

    Techniques: Biomarker Discovery, Expressing, Immunohistochemistry, Quantitative RT-PCR, Western Blot, Immunofluorescence